University College of Veterinary and Animal Sciences, Pakistan.
Title: Antiviral activities from selected Cholistani plants against common viral diseases of poultry birds
Miss Hina Ashraf is expertised in Plant Biotechnology and Medicinal plants. She is associated as a faculty member in the Department of Botany in Govt. Sadiq College Women University Bahawalpur Pakistan since 2014. She has supervised many research students and also has played an active role in winning projects for her department. She has expertise in the evaluation of antibacterial, antiviral, antioxidant antidiabetic and many other biological activities of Cholistani medicinal plants.
The idea of herbal medicine is getting popular day by day and demand for herbal products is continuously rising due to its usefulness, limited side effects and cost-effectiveness. Cholistani plants are reported as a rich source of antibacterial, antifungal, antiprotozoal, antioxidant and anticancer agents but their antiviral potentials were not explored before. The current study was designed to asses the antiviral capabilities of selected Cholistani plants. The whole plants were collected and authenticated by a taxonomist. Plants were shade dried and powdered form was used to make extracts with methanol, n-hexane, ethyl acetate and n-butanol. These extracts were concentrated by using a rotary evaporator and 0.5g each precipitate was dissolved in 1mL of the respective solvent to make final extract. All of these fractions were tested for their antiviral potentials through 9 to 11 days old chick embryonated eggs. HA test was performed to asses’ antiviral potential of each fraction against Avian Influenza virus H9N2 strain, New Castle Disease virus Lasota strain and Infectious bronchitis virus similarly, IHA test was used quantification of Infectious bursal disease virus. The order of antiviral activities of Cholistani plants was NDV>IBV>IBDV>AIV. In terms of antiviral activity from different fractions, the order of activity was n-butanol>ethyl acetate>methanol>n-hexane. The extracts of medicinal plants A. Aspera, N. procumbus antidotale, . compressa and S. fruticose were effective against all four poultry viruses. The low IC50values of these plant extracts confirm the very high antiviral potentials of these plants against above-mentioned poultry viruses. It is worth to mention that all extracts of A. aspera were positive against IBDV, which has no treatment in the market so far.